Efficient screening of retroviral cDNA expression libraries Proc Natl Acad Sci USA 1995 92: 9146–9150 pEF-BOS, a powerful mammalian expression vector Nucleic Acids Res 1990 18: 5332 STAT5 as a molecular regulator of proliferation, differentiation, and apoptosis in hematopoietic cells EMBO J 1999 18: 4754–4765 Analysis of mutation in human cells by using an Epstein–Barr virus shuttle system Mol Cell Biol 1987 7: 379–387 Improved FACS-Gal: flow cytometric analysis and sorting of viable eukaryotic cells expressing reporter gene constructs Cytometry 1991 12: 291–301ĭubridge RB, Tang P, Hsia HC. The encephalomyocarditis virus internal ribosome entry site allows efficient coexpression of two genes from a recombinant provirus in cultured cells and in embryo Mol Cell Biol 1991 11: 5848–5859įiering SN et al. Application of retrovirus-mediated expression cloning Exp Hematol 1996 24: 324–329 Production of high-titer helper-free retroviruses by transient transfection Proc Natl Acad Sci USA 1993 90: 8392–8396 Pear WS, Nolan GP, Scott ML, Baltimore D. Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production Mol Cell Biol 1986 6: 2983–2902 In addition, as we used only the coding sequences of viral structural genes to avoid inclusion of unnecessary retrovirus sequences in the packaging constructs, the probability of generating the replication competent retroviruses (RCR) by recombination can virtually be ruled out. Plat-E cells can stably produce retroviruses with an average titer of 1 × 10 7/ml for at least 4 months. To maintain the high titers of retroviruses under drug selection pressure, we inserted the IRES (internal ribosome entry site) sequence between the gene encoding gag-pol or env, and the gene encoding a selectable marker in the packaging constructs. Conventional packaging constructs made use of the promoter of MuLV-LTR for expression of viral structural genes gag-pol and env, while our packaging constructs utilized the EF1α promoter, which is 100-fold more potent than the MuLV-LTR in 293T cells in combination with the Kozak's consensus sequence upstream of the initiation codon resulting in high expression of virus structural proteins in Plat-E cells. The novel packaging constructs utilized in establishment of Plat-E ensure high and stable expression of viral structural proteins. Plat-E is superior to existing packaging cell lines regarding efficiency, stability and safety. He's probably going to be indicted three or four times more.A potent retrovirus packaging cell line named Platinum-E (Plat-E) was generated based on the 293T cell line. Mr Zogby went on: "It's hard to see, unless he falls flat on his face somehow - I mean, he's been impeached twice, he's been indicted once. But Democrats ought to be very, very careful, Trump did win in 2016." "On the other hand, Democrats should be careful what they wish for because yes, there is some evidence that in the national electorate Donald Trump has been damaged. He has solidified that - he's their spokesperson. Just click on blurred photo or video below to open.
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I suspect his numbers will probably go up just a bit more too now that the public is getting a full dose of this." "This is already helping him - and not only among Republican voters. Just two weeks earlier, it had been 14 points or so. Mr Zogby said: "The only two nationwide polls of Republicans show that his lead over his prime challenger in the presidential nomination, Ron DeSantis, has gone up to 31 points. He added that it is "close to inevitable" that Trump will secure the Republican presidential nomination for 2024 - rather than his closest challenger, Ron DeSantis. John Zogby, a US pollster, has told Sky News that "at least in the short run" Donald Trump's indictment will likely help his somewhat stilted political career.
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